کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2075673 | 1079360 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A highly selective whole cell biocatalysis method for the production of two major bioactive conjugated linoleic acid isomers
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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![عکس صفحه اول مقاله: A highly selective whole cell biocatalysis method for the production of two major bioactive conjugated linoleic acid isomers A highly selective whole cell biocatalysis method for the production of two major bioactive conjugated linoleic acid isomers](/preview/png/2075673.png)
چکیده انگلیسی
Conjugated linoleic acid (CLA) is well known potential anticarcinogenic, antiatherogenic and a number of other therapeutic qualities bearing substance. In the present study we have isolated Lactobacillus plantarum from local dairy buffalo milk and used as a whole cell biocatalyst, for selective CLA isomer production from linoleic acid (LA). Quantitative analysis revealed that the cis-9, trans-11 CLA comprised of 51% (w/w), while trans-10, cis-12 CLA accounted for 49% (w/w) of total CLA produced by L. plantarum. The product formation and quantitation was monitored by the spectrophotometric method and the individual isomers separation was done with GC-FID. After optimization of biotransformation conditions such as pH, LA concentration, effect of preincubation, fermentation time; the highest CLA production (19.07% conversion) was achieved at 120 h in the presence of 1.62 mg mlâ1 of LA. The results revealed that the bacterium is an efficient biocatalyst for the production of two major bioactive CLA isomers using LA as a substrate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 2, Issue 4, October 2013, Pages 328-332
Journal: Biocatalysis and Agricultural Biotechnology - Volume 2, Issue 4, October 2013, Pages 328-332
نویسندگان
Abid Ali Khaskheli, Farah N. Talpur, Ayhan S. Demir, Aysun Cebeci, Sana Jawaid,