Change of Hydrolase Activity in Germinating Seeds of trxS Transgenic Barley

Genetic modification of barley variety can be an efficient way to improve beer quality. The objective of this study was to understand the effect of trxS gene on hydrolases activities in transgenic and nontransgenic barley seeds. The results showed that α-amylase, free β-amylase, and limit dextrinase activity were increased in transgenic seeds when compared with nontransgenic seeds. Sulfhydryl content of protein in transgenic seeds was also higher than in nontransgenic seeds, suggesting that trxS gene could express in barley seeds, which opens a new way for breeding new barley varieties to improve beer quality.