کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2078945 1545063 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impact of subculture cycles and inoculum sizes on suspension cultures of Vitis vinifera
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Impact of subculture cycles and inoculum sizes on suspension cultures of Vitis vinifera
چکیده انگلیسی
The commercial application of plant cell cultures is often hindered because of the instability of secondary metabolite biosynthesis, where the metabolite yield fluctuates and declines dramatically over the subculture cycles. This study proposed that such instability is due to the fluctuations of culture variables. To validate this hypothesis, the effects of the fluctuations of two culture variables (subculture cycle and inoculum size) on the biomass, anthocyanin biosynthesis, and intracellular carbon, nitrogen and phosphate during 10 continuous subculture cycles were investigated. The subculture cycle was fluctuated for 12 h over a 7-day cycle (6.5, 7, and 7.5 d), and the inoculum size was fluctuated by 20 % on the wet-weight basis of 2.00 g (1.60, 2.00, and 2.40 g). It was found that all the measured culture parameters fluctuated over the 10 subculture cycles. The fluctuation in terms of inoculum sizes had a greater effect on the stability of anthocyanin biosynthesis in suspension cultures of V. vinifera. Among all the subculture conditions investigated, 7-d subculture cycle and 1.60-g inoculum size were the best for maintaining relatively stable anthocyanin production. The anthocyanin yield showed a negative correlation with intracellular sucrose content or intracellular total phosphate content.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 22, Issue 6, November 2006, Pages 984-989
نویسندگان
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