کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2079004 | 1545053 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression and Activity Analysis of the Catalytic Domain of PTP1B
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression and Activity Analysis of the Catalytic Domain of PTP1B Expression and Activity Analysis of the Catalytic Domain of PTP1B](/preview/png/2079004.png)
چکیده انگلیسی
The amino acid sequence (1-301aa) coding the human PTP1B catalytic domain (PTP1Bc) was obtained from the GenBank. The PTP1Bc gene was constructed by overlapping PCR, then inserted into vector pET-22b (+) and expressed efficiently in E. coli BL21 (DE3) under the optimization condition after IPTG induction. The proteins were expressed mainly as inclusion bodies with the yield of more than 30% of total bacterial proteins. The expressed products were purified through Ni2+-affinity chromatographic column. After purification, the purity of the proteins was more than 95%. The result of western blotting confirmed that the purified proteins could specially combine with anti-PTP1B antibody. The enzyme activities experiment showed that the protein had phosphatase activities. The gene construction, expression, and purification of PTP1Bc may provide basis for further study of its functions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 24, Issue 4, April 2008, Pages 553-557
Journal: Chinese Journal of Biotechnology - Volume 24, Issue 4, April 2008, Pages 553-557
نویسندگان
Shengyu Wang, Jianghua Yan, Yanglin Pan, Xuejun Li, Zhong Chen,