کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2079009 | 1545053 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Research on Orientedly Immobilized Urease via Concanavalin A
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, urease was immobilized on chitosan beads via a saccharide-concanavalin A binding. Concanavalin A (ConA) was immobilized on a pre-activated chitosan microsphere, and then oriented immobilization of urease was carried out based on the strong interaction between ConA and glycoprotein. The optimum immobilization conditions were as follows: glutaraldehyde concentration 3.5%, ConA concentration 1 mg/mL, ConA pH 7.0, and urease concentration 0.4 mg/mL. For orientedly immobilized urease, the highest activity was allowed at pH 5.0-6.0 and temperature 77°C, and the Michaelis constant (Km) was disclosed to be 11.76 mmol/L by Lineweaver-Burk plot. Compared with the free urease and the randomly immobilized urease, the optimum pH of the orientedly immobilized urease was smaller and the pH domain wider. Orientedly immobilized urease presents higher temperature resistance, higher affinity to the substrate, and higher stability of operation. Hence, oriented immobilization of urease via ConA can be a versatile tool for immobilization of proteins and offers great promise in clinical as well as industrial use.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 24, Issue 4, April 2008, Pages 617-621
Journal: Chinese Journal of Biotechnology - Volume 24, Issue 4, April 2008, Pages 617-621
نویسندگان
Jianqin Zhou, Shaohua Chen, Jianwen Wang,