Expression of AIV Subtype H5HA, H7HA and H9HA Hemagglutinin Gene in Pichia pastoris

The expression of the hemagglutinins of Avian Influenza Virus H5, H7 and H9 subtypes was studied in this article by Pichia pastoris, one of the eukaryotis expression systems. Three reconstructed expression plasmids and engineering strains, named pPIC9K-H5HA, pPIC9K-H7HA, pPIC9K-H9HA and GS115/pPIC9K-H5HA, GS115/pPIC9K-H7HA, GS115/pPIC9K-H9HA respectively, were obtained. The reconstructed yeast engineering strains were identified by MD and MM plate selecting and PCR. The induced interests proteins were examined by SDS-PAGE and Western-blotting, and the results showed that the interest genes were expressed exactly. This will be helpful in the future study of antigen detection and antibody detection kit, as well as in the development of subunit vaccines.