کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2079079 | 1545068 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immobilization of Lipase on Macroporous Resin and Its Application in Synthesis of Biodiesel in Low Aqueous Media
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immobilization of Lipase on Macroporous Resin and Its Application in Synthesis of Biodiesel in Low Aqueous Media Immobilization of Lipase on Macroporous Resin and Its Application in Synthesis of Biodiesel in Low Aqueous Media](/preview/png/2079079.png)
چکیده انگلیسی
Lipase from Candida sp. 99-125 was immobilized by physical adsorption onto macroporous resins. The results showed that the nonpolar resin NKA was the best carrier used in low aqueous media. 98.98 % of degree of immobilization could be achieved when the adsorption procedure was performed in the presence of heptane. The lipolytic activity and the apparent activity recovery of lipase adsorbed on resin in heptane were 4.07 and 3.43 times higher than that of lipase adsorbed in sodium phosphate buffer, respectively. The catalytic properties of immobilized lipase for production of biodiesel in low aqueous media were studied. Immobilized lipase displayed the highest activity when the crude enzyme/resin weight ratio was 1.92:1 and the water content (water/ oil weight ratio) was 15 % at 40 °C under pH 7.4. As lipase was adsorbed on NKA in heptane to produce biodiesel, the batch conversion rate reached 97.3 % when a three-step methanolysis protocol was used. After 19 consecutive batches, the conversion rate remained 70.2 %.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chinese Journal of Biotechnology - Volume 22, Issue 1, January 2006, Pages 114-118
Journal: Chinese Journal of Biotechnology - Volume 22, Issue 1, January 2006, Pages 114-118
نویسندگان
Yang GAO, Tian-Wei TAN, Kai-Li NIE, Fang WANG,