کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2083983 1545340 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chasing bacteria within the cells using levofloxacin-loaded hyaluronic acid nanohydrogels
ترجمه فارسی عنوان
تعقیب باکتری درون سلول ها با استفاده از نانو هیدروژل های اسید هیالورونیک لوافلوکساسین
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


• Hyaluronic acid-cholesterol nanohydrogels (NHs) were loaded with levofloxacine (LVF).
• LVF-NHs were active against P. aeruginosa and S. aureus.
• LVF-NHs eradicated bacteria in intracellular infections.

In the present work, an innovative approach based on the delivery of levofloxacin (LVF) from polysaccharide nanohydrogels for the treatment of bacterial intracellular infections is described. The nanohydrogels (NHs) were obtained by self-assembling of the hyaluronic acid-cholesterol amphiphilic chains in aqueous environment. LVF, a fluoroquinolone antibiotic scarcely efficient in intracellular infections, was entrapped within such NHs by nanoprecipitation, thus forming a drug delivery system (LVF-NHs) that was tested for its activity on different bacteria strains. The MIC values of levofloxacin-loaded nanohydrogels were determined for Staphylococcus aureus and Pseudomonas aeruginosa strains and compared to those obtained using free LVF. The intracellular antimicrobial activity of LVF-NHs and free LVF was compared on HeLa epithelial cell line infected by the above mentioned bacteria, and the increase in antibacterial efficacy of LVF-NHs with respect to that of free LVF was evidenced. The obtained results allow to conclude that this new approach can be considered as really promising method for intracellular infection treatments.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 87, Issue 3, August 2014, Pages 518–523
نویسندگان
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