کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2084110 1545365 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Understanding the mechanism of protamine in solid lipid nanoparticle-based lipofection: The importance of the entry pathway
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Understanding the mechanism of protamine in solid lipid nanoparticle-based lipofection: The importance of the entry pathway
چکیده انگلیسی

The aim of our study was to evaluate the effect of protamine on the transfection capacity of solid lipid nanoparticles (SLNs) by correlating it to the internalization mechanisms and intracellular trafficking of the vectors. Vectors were prepared with SLN, DNA, and protamine. ARPE-19 and HEK-293 cells were used for the evaluation of the formulations. Protamine induced a 6-fold increase in the transfection of SLNs in retinal cells due to the presence of nuclear localization signals (NLS), its protection capacity, and a shift in the internalization mechanism from caveolae/raft-mediated to clathrin-mediated endocytosis. However, protamine produced an almost complete inhibition of transfection in HEK-293 cells. In spite of the high DNA condensation capacity of protamine and its content in NLS, this does not always lead to an improvement in cell transfection since it may impair some of the limiting steps of the transfection processes.

Protamine induced an increase in the transfection of SLNs in retinal cells but a decrease in HEK-293 cells, which is related to the change in the internalization mechanism.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 79, Issue 3, November 2011, Pages 495–502
نویسندگان
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