کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2085510 | 1545378 | 2010 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effect of various additives and polymers on lysozyme release from PLGA microspheres prepared by an s/o/w emulsion technique Effect of various additives and polymers on lysozyme release from PLGA microspheres prepared by an s/o/w emulsion technique](/preview/png/2085510.png)
Incomplete protein release from PLGA-based microspheres due to protein interactions with the polymer is one of the main issues in the development of PLGA protein-loaded microspheres. In this study, a two-dimensional adsorption model was designed to rapidly assess the anti-adsorption effect of formulation components (additives, additives blended with the polymer or modified polymers). Lysozyme was chosen as a model protein because of its strong, non-specific adsorption on the PLGA surface. This study showed that PEGs, poloxamer 188 and BSA totally inhibited protein adsorption onto the PLGA37.5/25 layer. Similarly, it was emphasised that more hydrophilic polymers were less prone to protein adsorption. The correlation between this model and the in vitro release profile was made by microencapsulating lysozyme with a low loading in the presence of these excipients by a non-denaturing s/o/w encapsulation technique. The precipitation of lysozyme with the amphiphilic poloxamer 188 prior to encapsulation exhibited continuous release of active lysozyme over 3 weeks without any burst effect. To promote lysozyme release in the latter stage of release, a PLGA–PEG–PLGA tribloc copolymer was used; lysozyme was continuously released over 45 days in a biologically active form.
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 75, Issue 2, June 2010, Pages 128–136