کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2085515 1545378 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transdermal iontophoresis of dexamethasone sodium phosphate in vitro and in vivo: Effect of experimental parameters and skin type on drug stability and transport kinetics
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Transdermal iontophoresis of dexamethasone sodium phosphate in vitro and in vivo: Effect of experimental parameters and skin type on drug stability and transport kinetics
چکیده انگلیسی

The aim of this study was to investigate the cathodal iontophoresis of dexamethasone sodium phosphate (DEX-P) in vitro and in vivo and to determine the feasibility of delivering therapeutic amounts of the drug for the treatment of chemotherapy-induced emesis. Stability studies, performed to investigate the susceptibility of the phosphate ester linkage to hydrolysis, confirmed that conversion of DEX-P to dexamethasone (DEX) upon exposure to samples of human, porcine and rat dermis for 7 h was limited (82.2 ± 0.4%, 72.5 ± 4.8% and 78.6 ± 6.0% remained intact) and did not point to any major inter-species differences. Iontophoretic transport of DEX-P across dermatomed porcine skin (0.75 mm thickness) was studied in vitro as a function of concentration (10, 20, 40 mM) and current density (0.1, 0.3, 0.5 mA cm−2) using flow-through diffusion cells. Increasing concentration of DEX-P from 10 to 40 mM resulted in a ∼4-fold increase in cumulative permeation (35.65 ± 23.20 and 137.90 ± 53.90 μg cm−2, respectively). Good linearity was also observed between DEX-P flux and the applied current density (id; 0.1, 0.3, 0.5 mA cm−2; JDEX (μg cm2 h−1) = 237.98 id − 21.32, r2 = 0.96). Moreover, separation of the DEX-P formulation from the cathode compartment by means of a salt bridge – hence removing competition from Cl− ions generated at the cathode – produced a 2-fold increase in steady-state iontophoretic flux (40 mM, 0.3 mA cm−2; 20.98 ± 7.96 and 41.82 ± 11.98 μg cm−2 h−1, respectively).Pharmacokinetic parameters were determined in Wistar rats (40 mM DEX-P; 0.5 mA cm−2 for 5 h with Ag/AgCl electrodes and salt bridges). Results showed that DEX-P was almost completely converted to DEX in the bloodstream, and significant DEX levels were achieved rapidly. The flux across rat skin in vivo (1.66 ± 0.20 μg cm−2 min−1), calculated from the input rate, was not statistically different from the flux obtained in vitro across dermatomed porcine skin (1.79 ± 0.49 μg cm−2 min−1). The results suggest that DEX-P delivery rates would be sufficient for the management of chemotherapy-induced emesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 75, Issue 2, June 2010, Pages 173–178
نویسندگان
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