کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2086165 1545507 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pola Produksi Ajmalisin dari Kultur Agregat Sel Catharanthus roseus (L.) G. Don. dalam Bioreaktor Airlift
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Pola Produksi Ajmalisin dari Kultur Agregat Sel Catharanthus roseus (L.) G. Don. dalam Bioreaktor Airlift
چکیده انگلیسی

A research has been conducted to optimize the rate of aeration and initial weight of cell aggregates in the production of ajmalicine in Catharanthus roseus cell culture in airlift bioreactor. Catharanthus roseus culture were grown in Zenk medium with the addition of 2.50 × 10−6 M naphthalene acetic acid (NAA) and 10−5 M benzyl amino purine (BAP). Cell aggregates were sub-cultured two times before transferring 20 and 30 g/fw of cell aggregates into bioreactor, respectively, and aerated with the rate of 0.25 l min−1 and 0.34 l min−1, respectively. The pattern of ajmalicine production in bioreactor were observed in every three days within 24 days. Qualitative and quantitative analysis were conducted using HPLC connected to Cromatopac CL-7A Plus. The results showed that the cell aggregates and medium contain ajmalicine. The highest concentration was obtained in combination of 30 g/fw and 0.34 l min−1 aeration compare to 20 g/fw – 0.25 l min−1, 20 g/fw – 0.34 l min−1, as well as 30 g/fw – 0.25 l min−1. The highest ajmalicine content in cell aggregates was obtained on the 12 days (79.23 µg g−1) whilst in medium was obtained in the 18th days (981.15 µg l−1).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: HAYATI Journal of Biosciences - Volume 13, Issue 4, December 2006, Pages 161–165
نویسندگان
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