Enzyme-assisted extraction processing from oilseeds: Principle, processing and application

• EAEP is an efficient, environmentally friendly method to produce edible oil.
• Process of EAEP is notified constantly by users.
• Application of EAEP is transforming from laboratory scale to industrial scale.

Enzyme-assisted extraction processing (EAEP), feasible alternative to screw pressing and organic solvent extraction technologies, is a promising method for the simultaneous extraction of oil and protein from oilseed. This method incorporates comminuting, extraction buffers and enzymes to allow production of a range of oils and proteins although different challenges appear during the process. The demand for acceptable and high free oil yields and purities of protein is always incompatible in many processes. This review article covers technological aspects of EAEP, and discusses the application of enzymes in pretreatment, extraction and demulsification, and explores the quality characteristics and safety of the oils and proteins obtained, focusing particularly on recent application of EAEP at the laboratory and industrial scale.Industrial relevance1.Enzymes are widely used on an industrial scale but mainly as catalysts, and the applications mainly concerning the synthesis of pharmaceuticals, compounds for chemistry of specialty, polymers, etc.2.At the industrial scale, one advantage of EAEP is environment benefit because it can avoid the risk of organic solvents and particularly hexane.3.The quality of the products produced by the smooth process is usually higher.4.EAEP technique is considered as an alternative method to produce valuable products without loss of quality at moderate conditions.5.Compared with solvent extraction, EAEP is more eco-friendly. Besides that, one of the main advantages of EAEP is the specificity of enzymes.