Development of biocompatible nanogel for sustained drug release by overcoming the blood brain barrier in zebrafish model

- Delivery of drug to the zebrafish brain was achieved by overcoming the blood-brain barrier.
- PNIPAM nanogel had prepared and functionalized with polysorbate 80 for sustained donepezil delivery.
- Acetylcholineesterase inhibition in zebrafish brain had confirmed the drug release by donepezil conjugated nanogel.
- Concentration dependent swim behavior and neuronal biocompatibility is reported in adult zebrafish.
- Therapeutic efficiency of the donepezil conjugated nanogel was studied through cardiac and phenotypic assessment in zebrafish embryos.