Plant-based production of two chimeric monoclonal IgG antibodies directed against immunodominant epitopes of Vibrio cholerae lipopolysaccharide

• Ongoing cholera vaccine trials lack available standards for serum antibody analysis.
• We produced two chimeric IgG1 monoclonal antibodies using a Nicotiana benthamiana-based system.
• 2D6 IgG1 recognizes the immunodominant Ogawa O-polysaccharide antigen.
• ZAC-3 IgG1 recognizes core/lipid A moiety of Ogawa and Inaba lipopolysaccharides.
• 2D6 and ZAC-3 will serve as important reagents for the cholera research community.

We have produced and characterized two chimeric human IgG1 monoclonal antibodies that bind different immunodominant epitopes on Vibrio cholerae lipopolysaccharide (LPS). MAb 2D6 IgG1 recognizes Ogawa O-polysaccharide antigen, while mAb ZAC-3 IgG1 recognizes core/lipid A moiety of Ogawa and Inaba LPS. Both antibodies were expressed using a Nicotiana benthamiana-based rapid antibody-manufacturing platform (RAMP) and evaluated in vitro for activities associated with immunity to V. cholerae, including vibriocidal activity, bacterial agglutination and motility arrest.