کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2088425 | 1545735 | 2011 | 6 صفحه PDF | دانلود رایگان |
It is important to know the contribution of specific allergens to a complex allergenic extract and to have a dependable method to assess the effector activity of an extract specifically depleted of that allergen. We have previously shown that removal of the major peanut allergen, Ara h 2, from a crude peanut extract (CPE) minimally altered the effector activity of the extract. Here we describe in detail the methodology used to generate specific rabbit anti-peptide antibodies to remove a related peanut allergen, Ara h 6, from CPE and describe an improvement in the RBL SX-38 cell assay used to assess the effector activity of treated extracts. Our results show that although Ara h 2 and Ara h 6 can be selectively removed from a CPE, removal of each alone from a CPE had no significant effect on the effector activity. However, removal of Ara h 2 and Ara h 6 together significantly reduced the effector activity of CPE.
► Immunodepletion of Ara h 2 and Ara h 6 from a crude peanut extract (CPE).
► Improvement in the RBL SX-38 cell assay used to assess peanut allergens.
► Removal of Ara h 2 and Ara h 6 together reduced the effector activity of a CPE.
Journal: Journal of Immunological Methods - Volume 372, Issues 1–2, 30 September 2011, Pages 65–70