کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2088808 | 1545760 | 2009 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Isolation of functional human regulatory T cells (Treg) from the peripheral blood based on the CD39 expression Isolation of functional human regulatory T cells (Treg) from the peripheral blood based on the CD39 expression](/preview/png/2088808.png)
Human regulatory T cells (Treg) have been variously defined as CD4+CD25+, CD4+CD25high or CD4+CD25highFOXP3+ cells which are responsible for maintaining peripheral tolerance. Their isolation from human peripheral blood or tissues depends on the expression level of CD25(IL-2Rα) — a surface marker which is also expressed on activated effector helper T cells. CD39, a cell surface associated ectonucleotidase, can be used to purify Treg with strong suppressor functions. The CD4+CD39+ T cells catalyze cleavage of adenosine triphosphate (ATP) to adenosine monophosphate (AMP), which is then further cleaved to adenosine. CD4+CD39+ T cells largely overlap with CD4+CD25highFOXP3+ but not CD4+CD25+ T cell subset, and mediate equally potent immune suppression. Thus, CD39 surface marker can be successfully used for routine isolation of functionally-active human Treg from the peripheral blood of healthy donors or patients with cancer for studies of their role in health and disease.
Journal: Journal of Immunological Methods - Volume 346, Issues 1–2, 31 July 2009, Pages 55–63