کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2089358 | 1545787 | 2006 | 8 صفحه PDF | دانلود رایگان |
Intestinal inflammation in mice is most frequently assessed by histology or FACS, processes that necessitate sacrificing mice. We developed a lactoferrin ELISA for murine feces to quantify intestinal inflammation in mice with enteric infections or colitis. Levels of fecal lactoferrin, a protein secreted by activated neutrophils, were consistent with neutrophil infiltration as assessed by histology, indicating that this fecal lactoferrin ELISA is a good alternative to histology. The fecal lactoferrin ELISA provides a non-invasive, quantitative assessment of intestinal inflammation, which should facilitate longitudinal studies of the development of and/or therapies reducing intestinal inflammation in individual mice and reduce the number of mice needed for such studies.
Journal: Journal of Immunological Methods - Volume 313, Issues 1–2, 30 June 2006, Pages 183–190