کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2089591 | 1545788 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Improved preparation of class I HLA tetramers and their use in detecting CMV-specific CTL Improved preparation of class I HLA tetramers and their use in detecting CMV-specific CTL](/preview/png/2089591.png)
Different methods were used to prepare HLA tetramers and the yields of each method were compared. Our results indicate that preliminary refolding of the heavy chain (Hc) and light chain (β2m) yields more monomer than the typical conventional method with urea-solubilized Hc and β2m. We then used the corresponding tetramers to detect cytomegalovirus (CMV)-specific cytotoxic T lymphocytes (CTL). Increasing data suggest that the adoptive transfer of CMV-specific CTL constitutes an effective strategy against CMV infections. We designed a method that efficiently induces CMV-specific CTL to a higher frequency in vitro than is currently achieved. This method increased the percentage of CMV-specific CTL from below 1% to 20% of PBL, accounting for more than 40% of CD8+ T cells. Successful HLA tetramer preparation provides the basis for the subsequent detection of CMV-specific CTL in clinical applications.
Journal: Journal of Immunological Methods - Volume 312, Issues 1–2, 30 May 2006, Pages 148–156