کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2089682 1545914 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of ribotyping and sequence-based typing for discriminating among isolates of Bordetella bronchiseptica
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Comparison of ribotyping and sequence-based typing for discriminating among isolates of Bordetella bronchiseptica
چکیده انگلیسی


• PvuII ribotyping and MLST are equally discriminatory for B. bronchiseptica.
• MLST offers technical and practical advantages as compared to ribotyping.
• Isolates can often be further differentiated based on prn repeat region sequences.
• Combined MLST/prn repeat region typing offers maximum resolution and repeatability.
• A valuable approach for studying the evolution and epidemiology of the bacterium.

PvuII ribotyping and MLST are each highly discriminatory methods for genotyping Bordetella bronchiseptica, but a direct comparison between these approaches has not been undertaken. The goal of this study was to directly compare the discriminatory power of PvuII ribotyping and MLST, using a single set of geographically and genetically diverse strains, and to determine whether subtyping based on repeat region sequences of the pertactin gene (prn) provides additional resolution. One hundred twenty-two isolates were analyzed, representing 11 mammalian or avian hosts, sourced from the United States, Europe, Israel and Australia. Thirty-two ribotype patterns were identified; one isolate could not be typed. In comparison, all isolates were typeable by MLST and a total of 30 sequence types was identified. An analysis based on Simpson's Index of Diversity (SID) revealed that ribotyping and MLST are nearly equally discriminatory, with SIDs of 0.920 for ribotyping and 0.919 for MLST. Nonetheless, for ten ribotypes and eight MLST sequence types, the alternative method discriminates among isolates that otherwise type identically. Pairing prn repeat region typing with ribotyping yielded 54 genotypes and increased the SID to 0.954. Repeat region typing combined with MLST resulted in 47 genotypes and an SID of 0.944. Given the technical and practical advantages of MLST over ribotyping, and the nominal difference in their SIDs, we conclude MLST is the preferred primary typing tool. We recommend the combination of MLST and prn repeat region typing as a high-resolution, objective and standardized approach valuable for investigating the population structure and epidemiology of B. bronchiseptica.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 129, October 2016, Pages 117–126
نویسندگان
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