Evaluation of DiversiLab®, MLST and PFGE typing for discriminating clinical Enterococcus faecium isolates

• We compiled a set of 65 representative Enterococcus faecium/VRE isolates.
• Isolates were typed by Diversilab, PFGE and MLST.
• Performance, concordance and discriminatory power of all three methods were compared.

We evaluated and critically assessed the performance and discriminatory power of a rep-PCR based commercial test DiversiLab® Enterococcus kit (bioMerieux) for typing a set of 65 representative isolates of Enterococcus faecium/VRE and compared it to state-of-the-art typing techniques such as PFGE and MLST.