A new method for yeast phagocytosis analysis by flow cytometry

• New simple and fast flow cytometry method for yeast species phagocytosis
• Different Candida species present different interactions with macrophages.
• Quenching effect of PI over Sytox Green fluorescence of the labeled yeast cells

Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells.