Whole-genome mapping for high-resolution genotyping of Pseudomonas aeruginosa

• Whole genome mapping (WGM) was used to investigate 32 P. aeruginosa isolates.
• Bacterial strains included metallo beta-lactamase outbreak isolates.
• Sequence data was also obtained for 12 isolates from GenBank.
• WGM analysis revealed 3 genetic clusters.
• WGM is an alternative to current bacterial genotyping techniques.

A variety of molecular typing techniques have been developed to investigate the clonal relationship among bacterial isolates, including those associated with nosocomial infections. In this study, the authors evaluated whole-genome mapping as a tool to investigate the genetic relatedness between Pseudomonas aeruginosa isolates, including metallo beta-lactamase-positive outbreak isolates.