Real-time PCR detection of Brucella spp. DNA in lesions and viscera of bovine carcasses

• Our aim was to support the detection of Brucella spp. from lesions in cattle.
• We associated the Real-time PCR assay with FTA® Elute cards for diagnosis.
• It can be considered as an important diagnostic tool for use in slaughterhouses.

This study reports a real-time PCR assay for the detection of Brucella spp. associated with the FTA® Elute method in lesions observed during sanitary inspections in beef slaughter. Of the total 276 samples, 78 (28.3%) tested positive and 198 (71.7%) negative for Brucella spp. The real-time PCR technique associated with the FTA® Elute method proved to be an important tool for the diagnosis, judgment about and disposal of carcasses and viscera of slaughtered animals.