کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2090010 | 1545944 | 2014 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rapid and improved technique for scanning electron microscopy of actinomycetes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
• We report a simple and rapid method for preparation of actinomycete cultures for SEM.
• Actinomycete cultures were grown on glass wool, a chemically inert support.
• No chemical fixatives or dehydrating agents were used.
• Cultures were dehydrated by lyophilization.
• High resolution SEM images of Streptomyces spp. were obtained.
A simple and rapid method for the preparation of actinomycete cultures before scanning electron microscopy (SEM) is reported. Streptomyces strains were grown in starch casein broth with sterile glass wool as support. The cultures were dehydrated by lyophilization eliminating the use of chemical fixatives and dehydrating agents.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 99, April 2014, Pages 54–57
Journal: Journal of Microbiological Methods - Volume 99, April 2014, Pages 54–57
نویسندگان
Divya Prakash, N.N. Nawani,