کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090036 1081467 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assessment of a new Bacteroidales marker targeting North American beaver (Castor canadensis) fecal pollution by real-time PCR
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Assessment of a new Bacteroidales marker targeting North American beaver (Castor canadensis) fecal pollution by real-time PCR
چکیده انگلیسی


• Beaver can contain human pathogens.
• Primers and probe have been design to detect beaver fecal pollution by real-time PCR.
• System shown high specificity and high sensitivity.
• Beaver fecal pollution was successfully highlighted in Canadian environmental water.

In many settings wildlife can be a significant source of fecal pathogen input into surface water. The North American beaver (Castor canadensis) is a zoonotic reservoir for several human pathogens including Cryptosporidium spp. and Giardia spp. In order to specifically detect fecal pollution by beavers, we have developed and validated a beaver-specific Bacteroidales marker, designated Beapol01, based on the 16S rRNA gene. The marker is suitable for quantifying pollution using real-time PCR. The specificity and sensitivity of the marker was excellent, Beaver signal was detected in water of a mixed-activity watershed harbouring this rodent. Overall, Beapol01 will be useful for a better understanding of fecal source inputs in drainage basins inhabited by the beaver.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 95, Issue 2, November 2013, Pages 201–206
نویسندگان
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