کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2090036 | 1081467 | 2013 | 6 صفحه PDF | دانلود رایگان |

• Beaver can contain human pathogens.
• Primers and probe have been design to detect beaver fecal pollution by real-time PCR.
• System shown high specificity and high sensitivity.
• Beaver fecal pollution was successfully highlighted in Canadian environmental water.
In many settings wildlife can be a significant source of fecal pathogen input into surface water. The North American beaver (Castor canadensis) is a zoonotic reservoir for several human pathogens including Cryptosporidium spp. and Giardia spp. In order to specifically detect fecal pollution by beavers, we have developed and validated a beaver-specific Bacteroidales marker, designated Beapol01, based on the 16S rRNA gene. The marker is suitable for quantifying pollution using real-time PCR. The specificity and sensitivity of the marker was excellent, Beaver signal was detected in water of a mixed-activity watershed harbouring this rodent. Overall, Beapol01 will be useful for a better understanding of fecal source inputs in drainage basins inhabited by the beaver.
Journal: Journal of Microbiological Methods - Volume 95, Issue 2, November 2013, Pages 201–206