کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2090191 | 1545948 | 2012 | 4 صفحه PDF | دانلود رایگان |
Centrifugation is a necessary concentrating step for the detection of Mycobacterium tuberculosis in a liquid culture. However, centrifugation is biologically hazardous and presents an obstacle in the development of an automated culture system. A bead-based bacterial concentration method, TRICORE, was recently developed by Genetein Co., Ltd. We compared the efficacy of TRICORE and conventional centrifugation for concentrating M. tuberculosis in clinical sputum specimens by using liquid and solid culture systems. Among 90 pretreated clinical sputum specimens, 51 (57.3%) and 55 (61.8%) M. tuberculosis isolates were recovered by the MGIT culture system by using the centrifugation and TRICORE methods, respectively (chi‐square test, p = 0.5413). The detection time for the centrifugation method was 359.3 ± 117.0 h, while that for the bead-based concentration method was 377.6 ± 162.3 h (p = 0.5637). However, the number of colonies recovered on solid media were significantly higher with the TRICORE method (p = 0.003). In particular, among the smear-negative specimens, culture positivity of the TRICORE method was 39.6%, while that of the centrifugation method was 15.1%. The TRICORE bead-based concentration method was considered equivalent to centrifugation and enabled efficient collection of paucibacillary specimens in solution. Thus, the new noncentrifugation concentration method could yield more positive culture results.
► A noncentrifugation concentration method for M. tuberculosis was developed.
► Bead-based concentration method was evaluated for culture efficiency.
► Bead-based method is almost equivalent to centrifugation for liquid culture.
► Bead-based method shows higher sensitivity than centrifugation on solid media.
Journal: Journal of Microbiological Methods - Volume 90, Issue 3, September 2012, Pages 152–155