Magnetic capture hybridization of Batrachochytrium dendrobatidis genomic DNA

We hybridized biotinylated probes that anneal at multiple locations throughout the Batrachochytrium dendrobatidis (Bd) genome to selectively capture Bd genomic DNA (gDNA) by binding the probe-gDNA complex to streptavidin coated magnetic beads. We then whole genome amplified the captured gDNA. This method extends the usefulness of field-collected swabs for downstream PCR-based genomic applications.

► Batrachochytrium dendrobatidis (Bd) is responsible for massive amphibian declines.
► Low template concentrations and PCR inhibitors limit population studies of Bd.
► We combine magnetic capture hybridization with whole genome amplification.
► We recover Bd genetic material from low template frog skin swabs.
► This method will extend the usefulness of nonconsumptive field-collected swabs.