کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2090192 | 1545948 | 2012 | 4 صفحه PDF | دانلود رایگان |
We hybridized biotinylated probes that anneal at multiple locations throughout the Batrachochytrium dendrobatidis (Bd) genome to selectively capture Bd genomic DNA (gDNA) by binding the probe-gDNA complex to streptavidin coated magnetic beads. We then whole genome amplified the captured gDNA. This method extends the usefulness of field-collected swabs for downstream PCR-based genomic applications.
► Batrachochytrium dendrobatidis (Bd) is responsible for massive amphibian declines.
► Low template concentrations and PCR inhibitors limit population studies of Bd.
► We combine magnetic capture hybridization with whole genome amplification.
► We recover Bd genetic material from low template frog skin swabs.
► This method will extend the usefulness of nonconsumptive field-collected swabs.
Journal: Journal of Microbiological Methods - Volume 90, Issue 3, September 2012, Pages 156–159