Fine visualization of filamentous structures in the bacterial cytoplasm

A new method was established for fine visualization of bacterial subcelluar filamentous structures by freezing the bacterial cells to displace cytoplasmic matrix granules to the periphery. This method was successfully applied in immunoelectron microscopy and electron microscopic tomography, and should be applicable for further studies of bacterial architecture and nanotransportation.

► We improved the protocol to observe cytoplasmic filaments in bacteria.
► It was to displace cytoplasmic granules to form an ice window in the cell.
► It could be applied to immunoelectron microscopy (EM) and EM tomography.