کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090383 1081499 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Diagnosis of Helicobacter pylori bacterial infections using a voltammetric biosensor
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Diagnosis of Helicobacter pylori bacterial infections using a voltammetric biosensor
چکیده انگلیسی

The voltammetric assay of Helicobacter pylori DNA was investigated using a bismuth-immobilized carbon nanotube electrode (BCNE). The analytical cyclic voltammetry (CV) peak potential was obtained at a 0.4 V reduction scan, where the diagnostic optimum square-wave (SW) stripping working range was achieved at 0.72–7.92 μg/mL H. pylori DNA (11 points). A relative standard deviation of 1.68% (RSD, n = 5) was obtained with 3.2 mg/mL H. pylori DNA using a 240 s accumulation time. Under optimum conditions, detection limit was 0.06 μg/mL. The developed sensors can be used for clinical application in the 15th doubted human gastric tissues, since the patient's peak current increased a hundred times more than the negative healthy tissue did. The sensing time obtained was only two minutes, and the process was simpler compared to common PCR amplification and electrophoresis photometric detection systems.


► The voltammetric assay of Helicobacter pylori DNA was investigated using a bismuth-immobilized carbon nanotube electrode.
► Peak potential was obtained at a 0.4 V reduction scan.
► Stripping working range was achieved at 0.72–7.92 ug/mL HP DNA.
► A relative standard deviation of 1.68% (RSD, n = 5) with 3.2 mg/mL HP DNA using a 240 s accumulation time.
► It can also be used in other fields requiring human DNA diagnostic analysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 87, Issue 1, October 2011, Pages 44–48
نویسندگان
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