کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090392 1081499 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid O serogroup identification of the ten most clinically relevant STECs by Luminex microbead-based suspension array
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Rapid O serogroup identification of the ten most clinically relevant STECs by Luminex microbead-based suspension array
چکیده انگلیسی

Identification and serotyping of Shiga toxin-producing Escherichia coli during foodborne outbreaks can aid in matching clinical, food, and environmental isolates when trying to identify the source of illness and ultimately food contamination. Herein we describe a Luminex microbead-based suspension array to identify the O serogroup of the ten most clinically relevant STECs: O26, O45, O91, O103, O111, O113, O121, O128, O145, and O157. The use of PCR followed by Luminex xMAP® technology enables the detection of multiple analytes in a single multiplex reaction with high throughput capabilities. One hundred and fourteen STEC isolates were correctly identified with no false positives among forty-six other organisms using this assay. Assay performance was tested in multiple laboratories using a panel of eleven different STEC serogroups on the Bio-Plex 200 and MAGPIX instruments. The STEC microbead-based suspension array can be performed in a 96-well plate format for high throughput screening in less than 4 h. Furthermore, it is expandable, allowing for the addition of O serogroups should the need arise.


► Luminex microbead-based suspension array can identify the 10 most clinically relevant STECs.
► STEC Luminex assay correctly identified the O serogroup of 114 STEC isolates.
► STEC Luminex assay can be performed in high throughput 96 well format.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 87, Issue 1, October 2011, Pages 105–110
نویسندگان
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