کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090396 1081499 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Amplified fragment length polymorphism analysis of Listeria monocytogenes by Experion™ automated microfluidic electrophoresis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Amplified fragment length polymorphism analysis of Listeria monocytogenes by Experion™ automated microfluidic electrophoresis
چکیده انگلیسی

Fifty Listeria monocytogenes strains were genotyped by sAFLP and PCR products were separated by agarose gel and automated chip-based microfluidic electrophoresis. A high congruency of results was observed comparing the two techniques, although for some cultures a better separation of sAFLP fragments was achieved with microfluidic system, which proved to be a highly reliable and reproducible tool to improve the molecular typing of L. monocytogenes, requiring lower volumes of samples and reducing significantly analysis time.


► An automated chip-based microfluidic electrophoresis was evaluated for L. monocytogenes sAFLP.
► Results were compared with analysis based on agarose gel electrophoresis.
► Data from both electrophoresis methods were in agreement.
► Microfluidic electrophoresis was sensitive, accurate, reproducible, with shorter analysis time.
► The system represents a reliable tool for epidemiological surveillance of human listeriosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 87, Issue 1, October 2011, Pages 119–124
نویسندگان
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