کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2090396 | 1081499 | 2011 | 6 صفحه PDF | دانلود رایگان |

Fifty Listeria monocytogenes strains were genotyped by sAFLP and PCR products were separated by agarose gel and automated chip-based microfluidic electrophoresis. A high congruency of results was observed comparing the two techniques, although for some cultures a better separation of sAFLP fragments was achieved with microfluidic system, which proved to be a highly reliable and reproducible tool to improve the molecular typing of L. monocytogenes, requiring lower volumes of samples and reducing significantly analysis time.
► An automated chip-based microfluidic electrophoresis was evaluated for L. monocytogenes sAFLP.
► Results were compared with analysis based on agarose gel electrophoresis.
► Data from both electrophoresis methods were in agreement.
► Microfluidic electrophoresis was sensitive, accurate, reproducible, with shorter analysis time.
► The system represents a reliable tool for epidemiological surveillance of human listeriosis.
Journal: Journal of Microbiological Methods - Volume 87, Issue 1, October 2011, Pages 119–124