Co-culture of human gastric endoscopic biopsies with Helicobacter pylori: A simple method for studying early phases of bacteria–host interaction

Gastric biopsies obtained through endoscopy from patients uninfected by Helicobacter pylori were co-cultured with an H. pylori strain. According to tissue and H. pylori viability, interleukin 8 was increased in biopsy homogenate and supernatant after 12–36h culture. This simple method is suitable to investigate early phases of bacteria–host interaction.