کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090832 1081520 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid screen for epithelial internalization of Tn917-mutagenized Streptococcus pyogenes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Rapid screen for epithelial internalization of Tn917-mutagenized Streptococcus pyogenes
چکیده انگلیسی

Group A streptococci (GAS) cause a number of human diseases ranging from pharyngitis to necrotizing fasciitis. GAS are hypothesized to escape killing by either the immune system or beta lactam antibiotics by internalization into epithelial cells. A Tn917 library of transposon mutants was screened for capacity to invade and survive in human epithelial cells using a novel blood agar overlay method. Although the screen revealed that a majority of Tn917 insertions occurred within a 10kb region of the genome, GAS genes identified as essential for internalization into epithelial cells included ABC transporters, and DNA maintenance proteins, and citrate metabolism enzymes, underlining the importance of adaptation to the intracellular environment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 78, Issue 1, July 2009, Pages 34–39
نویسندگان
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