کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090853 1081521 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assessment of horizontal gene transfer in Lactic acid bacteria – A comparison of mating techniques with a view to optimising conjugation conditions
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Assessment of horizontal gene transfer in Lactic acid bacteria – A comparison of mating techniques with a view to optimising conjugation conditions
چکیده انگلیسی

Plate, filter and broth mating techniques were assessed over a range of pHs using three Lactococcus lactis donor strains (one with an erythromycin resistance marker and two with tetracycline resistance markers, all located on transferable genetic elements) and one L. lactis recipient strain. Transconjugants were confirmed using antibiotic selection, E-tests to determine MICs, PCR assays to detect the corresponding marker genes, DNA fingerprinting by pulsed-field gel electrophoresis (PFGE), and Southern blotting. Horizontal gene transfer (HGT) rates varied (ranging from 1.6 × 10− 1 to 2.3 × 10− 8). The general trend observed was plate > filter > broth, independent of pH. Our data suggests that standardisation of methodologies to be used to assess HGT, is warranted and would provide a meaningful assessment of the ability of commensal and other bacteria in different environments to transfer relevant markers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 77, Issue 1, April 2009, Pages 23–28
نویسندگان
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