کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090877 1081522 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enumeration of airborne bacteria and fungi using solid phase cytometry
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Enumeration of airborne bacteria and fungi using solid phase cytometry
چکیده انگلیسی

Conventional methods for the enumeration of airborne micro-organisms are inaccurate and time-consuming, hence the interest in novel approaches is increasing. In the present study, the use of solid phase cytometry (SPC) was evaluated for the enumeration of airborne micro-organisms. A 4 h SPC procedure based on viability staining was applied to samples from 50 locations and compared with an optimised culture-based method. Plate counts after air sampling were repeatable but strongly dependent on sampling volume. Samples with low or high microbial load were difficult to analyse using the culture-based method, unlike with SPC. Results show that SPC can be considered superior to the culture-based method because of its much higher dynamic range, its speed and its ability to enumerate not only culturable but all viable micro-organisms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 72, Issue 1, January 2008, Pages 12–19
نویسندگان
, , ,