کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090919 1081523 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel specific DNA marker in Saccharomyces bayanus for species identification of the Saccharomyces sensu stricto complex
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
A novel specific DNA marker in Saccharomyces bayanus for species identification of the Saccharomyces sensu stricto complex
چکیده انگلیسی

This study was based on RAPD fingerprinting for species identification of the Saccharomyces sensu stricto complex. 40 random primers were used for RAPD analysis. The results showed that one of these primers, OPT-18, produced a 974 bp species-specific band, which was only found in the tested S. bayanus. Afterward this specific fragment was isolated from agarose gel and ligated into vector for DNA sequencing. A pair of primer SpeOPT18Sbay-F2 and SpeOPT18Sbay-R2 were designed according to the cloned species-specific sequence, which was employed for PCR with the template DNA of the S. sensu stricto strains, single 779 bp species-specific band was only found in S. bayanus. Therefore, we conclude that our novel species DNA marker could be used to rapidly and accurately identify the species of S. bayanus from S. sensu stricto complex by direct PCR.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 75, Issue 3, December 2008, Pages 531–534
نویسندگان
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