کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090956 1081525 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A split-protein sensor for studying protein–protein interaction in mycobacteria
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
A split-protein sensor for studying protein–protein interaction in mycobacteria
چکیده انگلیسی

The study of protein function in living cells is an essential complement to genomics, yet method development does not always keep pace with sequencing. Experimental techniques for the genus mycobacteria are relatively underdeveloped, though seventeen genomes have been sequenced. “Split-Trp” is a split-protein sensor used to detect protein–protein interactions in tryptophan auxotrophic Saccharomyces cerevisiae, but the principles behind the sensor should allow it to function in a broad range of microbial hosts. Here we introduce Split-Trp to Escherichia coli and Mycobacterium smegmatis and demonstrate that this system is a simple assay for protein interaction in both organisms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 73, Issue 2, May 2008, Pages 79–84
نویسندگان
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