کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090969 1081525 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chromosomal integration of a binding domain:bait gene into yeast enhances detection in the two-hybrid system
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Chromosomal integration of a binding domain:bait gene into yeast enhances detection in the two-hybrid system
چکیده انگلیسی

We have developed a modified yeast two-hybrid system using the GAL4 transcription activator by integrating a BD:bait gene (GAL4 binding domain:bait gene) into the host chromosome. Locus-specific integration by homologous recombination and use of a strong transcription promoter enabled uniform expression of the integrated BD:bait gene in all host cells. Moreover, after introduction of an AD:prey (GAL4 activation domain:prey) plasmid, false positives were nearly undetectable and false negatives were decreased significantly. Using this improved method a cDNA library could be efficiently screened using roughly 1/10 of the materials required for conventional sequential two-hybrid screen.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 73, Issue 2, May 2008, Pages 179–184
نویسندگان
, ,