کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2091569 1081739 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Agarose stabilization of fragile biofilms for quantitative structure analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Agarose stabilization of fragile biofilms for quantitative structure analysis
چکیده انگلیسی

Agarose was used to stabilize fragile biofilms cultivated in parallel plate flow cells prior to imaging by confocal laser scanning microscopy. An essential element to the success of the procedure was the application of a ceramic heat pad to the flow cell to maintain agarose fluidity until the biofilm was enveloped. Quantitative digital image analysis demonstrated the effectiveness of this technique for generating reproducible measurements of a three-dimensional biofilm structure. The described method will also benefit researchers who transport their flow cell-cultivated biofilms to a core facility for imaging.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 81, Issue 2, May 2010, Pages 101–107
نویسندگان
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