کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2091931 1081756 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bioluminescence of Vibrio fischeri in continuous culture: Optimal conditions for stability and intensity of photoemission
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Bioluminescence of Vibrio fischeri in continuous culture: Optimal conditions for stability and intensity of photoemission
چکیده انگلیسی

Continuous cultivation of the bioluminescent bacterium, Vibrio fischeri NRRL-B-11177, in a fermenter provided a constant supply of bacteria in exponential growth phase. These bacteria may be used as stable bioindicators to measure perturbation of the metabolic state by physical and chemical agents. However, optimization of light emission necessitates careful choice of growth medium and culture operating conditions. Optimized conditions that supported long-term cultivation of V. fischeri NRRL-B-11177 with stable intense bioluminescence were: a dilution rate of 0.08–0.09 h− 1, air supply of 600 ml min− 1, stirring of 300–350 rpm at a constant incubation temperature within the range of 20 to 26 °C and pH 7.8. These conditions were as successful in a 500-ml as in a 10-ml fermenter. This system provided a reliable long-term (more than 1 month) continuous culture facility for the reproducible measurement of perturbation of V. fischeri by monitoring changes in luminescence.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 67, Issue 2, November 2006, Pages 321–329
نویسندگان
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