کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2091932 | 1081756 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Rapid laboratory detection of meningococcal disease outbreaks caused by serogroup C Neisseria meningitidis Rapid laboratory detection of meningococcal disease outbreaks caused by serogroup C Neisseria meningitidis](/preview/png/2091932.png)
Molecular subtyping is of significant importance to the recognition of outbreaks of meningococcal disease caused by serogroup C Neisseria meningitidis. We describe the application of multilocus variable number tandem repeat analysis (MLVA) for the molecular subtyping of N. meningitidis and compare its performance to that of pulsed-field gel electrophoresis (PFGE). For MLVA, a multiplex PCR assay targeting five variable number tandem repeat regions was developed and evaluated using a panel of sporadic and outbreak-associated serogroup C N. meningitidis isolates. MLVA was highly reproducible and provided results within 6 h. Overall, the discriminatory power of MLVA was equivalent to that of PFGE. The utilization of MLVA for subtyping N. meningitidis isolates provides a rapid and safer alternative to PFGE for identifying outbreaks of meningococcal disease. As such, it may provide public health officials with timely information that may minimize the spread of outbreak-related cases through prophylaxis.
Journal: Journal of Microbiological Methods - Volume 67, Issue 2, November 2006, Pages 330–338