کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2092829 1081819 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of a cytochrome c gene located at the gene cluster for chlorate respiration in Ideonella dechloratans
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Characterization of a cytochrome c gene located at the gene cluster for chlorate respiration in Ideonella dechloratans
چکیده انگلیسی

SummaryAnaerobic chlorate respiration requires electron transport from the bacterial inner membrane to the soluble periplasmic chlorate reductase. We have recently demonstrated that soluble c cytochromes function as electron carriers for chlorate reduction in Ideonella dechloratans ( Smedja Bäcklund et al. 2009). In the present work, we describe a gene encoding soluble c-type cytochrome [cyt; GenBank ID: EU768872] located close to the gene cluster for chlorate reduction in I. dechloratans. The predicted amino acid sequence does not match any of the peptide masses or partial sequences obtained earlier from periplasmic c cytochromes. The gene, without the predicted signal sequence, was expressed heterologously in E. coli and the recombinant protein was purified, refolded and reconstituted with heme. The reconstituted protein shows spectral properties characteristic for c cytochromes, with an absorption maximum at 553 nm for the alpha band in the reduced state. Pyridine hemochrome analysis demonstrates the formation of covalently bound heme.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 165, Issue 6, 20 August 2010, Pages 450–457
نویسندگان
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