کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2092998 1546001 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of cyclophosphamide on immune system and gut microbiota in mice
ترجمه فارسی عنوان
اثرات سیکلوفسفامید بر سیستم ایمنی و میکروبیوتاسیون روده در موش
کلمات کلیدی
سیکلوفسفامید، توالی انتخابی بالا، میکروبیولوژیک روده، فعالیت ایمنی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی

Cyclophosphamide (CP) is the most commonly used drug in autoimmune disease, cancer, blood and marrow transplantation. Recent data revealed that therapy efficacy of CP is gut microbiota-dependent. So, it is very important to understand how CP affects intestinal microbiota and immune function. In this study, the effects of CP on mice immuno-activity were firstly evaluated, then, the fecal microbiota from normal and CP-treated mice was compared, and the characteristic bacterial diversity and compositions were identified, using 454 pyrosequencing technology. The results showed that CP reduced the diversity and shifted the fecal microbiota composition. Specifically, CP treatment decreased the proportion of Bacteroidetes while increased the proportion of Firmictutes in the microbial community. Most importantly, specific microbiota signatures belonging to Bacteroides acidifaciens, Streptococcaceae and Alistipes were also identified, which would provide new insight into the efficacy and side effects in clinical usage of CP. This should be helpful for further demonstration of CP's action mechanism, development of personalized therapy strategies, and prediction of potential side effects related to various treatment regimens of CP.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 171, February 2015, Pages 97–106
نویسندگان
, ,