کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2093312 1081952 2016 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo
چکیده انگلیسی


• AGM explants contain two HSC types, BMP-activated and non-BMP-activated
• Non-BMP-activated HSCs are dependent on Hedgehog/VEGF
• Changes in the microenvironment ex vivo contribute to novel HSC composition

SummaryHematopoietic stem cells (HSC), the self-renewing cells of the adult blood differentiation hierarchy, are generated during embryonic stages. The first HSCs are produced in the aorta-gonad-mesonephros (AGM) region of the embryo through endothelial to a hematopoietic transition. BMP4 and Hedgehog affect their production and expansion, but it is unknown whether they act to affect the same HSCs. In this study using the BRE GFP reporter mouse strain that identifies BMP/Smad-activated cells, we find that the AGM harbors two types of adult-repopulating HSCs upon explant culture: One type is BMP-activated and the other is a non-BMP-activated HSC type that is indirectly controlled by Hedgehog signaling through the VEGF pathway. Transcriptomic analyses demonstrate that the two HSC types express distinct but overlapping genetic programs. These results revealing the bifurcation in HSC types at early embryonic stages in the AGM explant model suggest that their development is dependent upon the signaling molecules in the microenvironment.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 6, Issue 3, 8 March 2016, Pages 383–395
نویسندگان
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