کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2096160 1082155 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrical activation induces reactive oxygen species in porcine embryos
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Electrical activation induces reactive oxygen species in porcine embryos
چکیده انگلیسی

The objectives were to determine factors affecting generation of reactive oxygen species (ROS) in porcine embryos after electrical activation of oocytes, and the effects of an antioxidant and chemical agent on ROS generation. Greater ROS were induced by electrical activation compared to IVF (mean ± S.E.M., 14.6 ± 0.8 vs. 9.2 ± 0.4, P < 0.05). Furthermore, ROS generation in embryos after electrical activation was significantly increased by higher intensity and longer duration electrical pulses and by higher exogenous Ca2+ concentrations. Cleavage rate and blastocyst formation rate were not directly related to the level of ROS. Supplementation of the IVC medium with 0.5 mM glutathione (GSH) reduced ROS (9.2 ± 0.4 vs. 14.7 ± 0.9, P < 0.05). Treatment with the chemical activation agent, 6-dimethylaminopurine (6-DMAP) for 3 h did not induce further ROS generation in combination with electrical activation, but it improved blastocyst formation rate (53.8 ± 1.1 vs. 23.7 ± 3.5, P < 0.05). We concluded that generation of ROS should be considered for optimizing electrical activation and that supplementing an antioxidant or combining electrical and chemical activation induced lower ROS generation in electrically activated porcine embryos.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 70, Issue 7, 15 October 2008, Pages 1111–1118
نویسندگان
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