کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2096210 1082157 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quality and in vitro fertilizing ability of cryopreserved cat spermatozoa obtained by urethral catheterization after medetomidine administration
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Quality and in vitro fertilizing ability of cryopreserved cat spermatozoa obtained by urethral catheterization after medetomidine administration
چکیده انگلیسی

Quality and in vitro fertilizing ability of frozen-thawed cat semen collected by urethral catheterization (CT) or electroejaculation (EE) after medetomidine administration were compared. Sperm collection was performed by an urinary tomcat catheter and, 4 days apart, by electroejaculation from each of eight tomcats. Results showed that semen collected by CT was characterized by lower volume (10.5 ± 5.3 μL, P < 0.05), higher sperm concentration (1868.4 ± 999.8 × 106/mL, P < 0.05) and lower pH (7.0 ± 0.4, P < 0.05) than that collected by EE (67.1 ± 25.9 μL, 542.9 ± 577.9 × 106/mL, and 7.9 ± 0.4, respectively). Spermatozoa characteristics after thawing at 0, 3 and 6 h did not differ between the two methods of collection. Also cleavage rate and embryo production from oocytes fertilized with frozen-thawed spermatozoa collected by CT or EE showed no significant differences (P > 0.05). In conclusion, the results obtained in the present study indicate that good quality freezable semen can be collected from cats by urethral catheterization after medetomidine administration. This new method of semen collection appears very useful in practice and, compared with the electroejaculation protocol, permits to obtain semen samples characterized by a higher concentration of spermatozoa, lower total volume and lower pH.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 69, Issue 4, 1 March 2008, Pages 485–490
نویسندگان
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