کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2096631 1082174 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improved survival of vitrified porcine embryos after partial delipation through chemically stimulated lipolysis and inhibition of apoptosis
ترجمه فارسی عنوان
بقای بهبودی جنین های جنین روده ای پس از تجزیه جزئی از طریق لیپولیز شیمیایی تحریک شده و مهار آپوپتوز
کلمات کلیدی
جنین خوک، فریزر چربی های داخل سلولی، عوامل لیپولیتیک، فورسکولین
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی

Mechanical removal of intracellular lipids has been the most effective approach to increase the cryosurvival of porcine embryos. In this experiment, we tested the hypotheses that the cryosurvival of porcine embryos can be improved after partial delipation through chemically stimulated lipolysis and that the survival can be further improved by inhibition of apoptosis. Porcine embryos were produced in vitro using sow oocytes. On Day 5 of embryonic development, embryos were cultured in the presence of 10 μM forskolin for 24 h. On Day 6 blastocysts were vitrified using an open pulled straw (OPS) method and warmed blastocysts were cultured 18 h for them to recover. A caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-FMK) was used at 20 μM during vitrification and subsequent culture to inhibit apoptosis. A 2 × 2 × 2 factorial design experiment was conducted to examine the effect of chemical delipation, vitrification and apoptosis inhibition. We also measured the lipolytic activity of porcine embryos cultured with or without forskolin. Chemical delipation increased the cryosurvival of porcine embryos compared to the controls (71.2 ± 2.8% versus 37.1 ± 5.1%). Apoptosis inhibition increased the ability of blastocysts to fully recover (23.8 ± 3.1% versus 14.6 ± 4.3%). However, there was no interaction between chemical delipation and apoptosis inhibition. Lipolytic agent treatment increased the lipolytic activity of porcine blastocysts. In conclusion, cryosurvival of porcine embryos was improved by partial delipation through chemical stimulation of lipolysis or apoptosis inhibition.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 66, Issue 8, November 2006, Pages 2008–2016
نویسندگان
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