کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2096990 | 1082190 | 2005 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen](/preview/png/2096990.png)
Cryopreservation is associated with the production of reactive oxygen species which lead to lipid peroxidation of sperm membranes. The objective was to determine an α-tocopherol concentration capable of improving the quality of cryopreserved porcine semen. Boar spermatozoa frozen with 200, 500 or 1000 μg/mL α-tocopherol were thawed and incubated at 37 °C for 4 h. Routine parameters of semen quality, susceptibility to lipid peroxidation 2-thiobarbituric acid (TBARS) and oxygen uptake were evaluated. Motility was higher (P < 0.05) in samples treated with different concentrations of α-tocopherol up to 2 h of incubation. Viability and acrosome integrity significantly decreased during incubation (no significant differences between treatments). Two hundred micrograms per milliliter α-tocopherol protected spermatozoa against lipid peroxidation during incubation, but 1000 μg/mL failed to protect after 2 h of incubation. There was a negative association between TBARS and motility, suggesting that lipid peroxidation affected sperm motility. Both control and 200 μg/mL α-tocopherol samples preserved the capacity to generate oxidative energy up to 1 h of incubation. The addition of 200 μg/mL α-tocopherol in the semen extender could be useful to preserve boar spermatozoa against the oxidative stress generated by cryopreservation.
Journal: Theriogenology - Volume 63, Issue 8, May 2005, Pages 2126–2135