کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2097170 1082201 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Offspring derived from oocytes injected with rat sperm, frozen or freeze-dried without cryoprotection
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Offspring derived from oocytes injected with rat sperm, frozen or freeze-dried without cryoprotection
چکیده انگلیسی

Sperm preservation is a valuable technique for maintaining genetic resources in biomedical research. In the present study, 10 mM Tris–HCl and 1 mM EDTA (TE buffer; a simple solution without cryoprotection), was used to freeze or freeze-dry rat sperm. The results were compared with rat sperm frozen using a solution containing Equex STM and egg yolk. Sperm from Wistar and Sprague–Dawley (SD) rats were evaluated by injecting them individually into oocytes derived from the same strain. Of the oocytes that survived after sperm injection, more than 94% were fertilized in all treatments of both strains. In the Wistar rat, 27, 20, 43, and 30% of 2-cell embryos developed to blastocysts, and 35, 9, 11, and 14% of 2-cell embryos developed to offspring from oocytes injected with fresh, frozen (Equex STM/egg yolk), frozen (TE buffer), and freeze-dried sperm, respectively. Using the analagous sources of sperm in the SD rat, 45, 14, 27, and 7% of 2-cell embryos developed to blastocysts, and 22, 0, 14, and 4% of 2-cell embryos developed to offspring. These results demonstrated that rat sperm could be frozen or freeze-dried using TE buffer. We concluded that this simple preservation method, in which cryoprotection was not required, allowed sperm to be preserved efficiently with maintenance of their fertilizing ability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 68, Issue 7, 15 October 2007, Pages 1017–1021
نویسندگان
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